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Asian primates hit hard by ancient climate change
Fossil finds may explain why humans evolved in Africa, not Asia

By Bruce Bower
2:00pm, May 5, 2016

Fossil discoveries in southern China point to an evolutionary crossroads around 34 million years ago that resulted in humans evolving in Africa rather than Asia, scientists say.

A sharply cooler and drier climate at that time, combined with upheavals of landmasses that forged the Himalayas and the Tibetan Plateau, destroyed many tropical forests in Asia. That sent surviving primates scurrying south, say paleontologist Xijun Ni of the Chinese Academy of Sciences in Beijing and his colleagues. New Chinese finds provide the first fossil evidence that the forerunners of monkeys, apes and humans, also known as anthropoids, were then largely replaced in Asia by creatures related to modern lemurs, lorises and tarsiers, the researchers conclude in the May 6 Science.



Continue Reading -> Asian primates hit hard by ancient climate change | Science News

Journal Reference:
Xijun Ni, Qiang Li, Lüzhou Li, K. Christopher Beard. Oligocene primates from China reveal divergence between African and Asian primate evolution. Science, 2016 DOI: 10.1126/science.aaf2107
 
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Large-scale Chinese reactor design passes IAEA safety review
05 May 2016

China's CAP1400 reactor design has successfully passed the International Atomic Energy Agency's (IAEA's) Generic Reactor Safety Review (GRSR), the Shanghai Nuclear Engineering Research and Design Institute (SNERDI) announced today.

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An artist's impression of how a plant based on the CAP1400 could appear (Image: SNPTC)

The GRSR process reviews the completely- or partially-developed safety cases of new reactor designs that are not yet in the licensing stage. It involves an international team of experts evaluating design safety case claims against selected and applicable IAEA safety standards. The review is not a clearance process but a review of the quality of the safety documents identifying strengths, weaknesses and gaps.

SNERDI - a subsidiary of the State Nuclear Power Technology Corporation (SNPTC) - signed an agreement with the IAEA last July for a GRSR review of the CAP1400 design.

The company has now announced that the design successfully completed the GRSR review, with the IAEA submitting its final GRSR evaluation report on 27 April.

The CAP1400 is an enlarged version of the AP1000 pressurized water reactor developed from the Westinghouse original by SNPTC with consulting input from the Toshiba-owned company. As one of China's 16 strategic projects under its National Science and Technology Development Plan, the CAP1400 is intended to be deployed in large numbers across the country. The reactor design may also be exported.

In a statement, SNERDI said: "The successful completion of the IAEA Generic Reactor Safety Review marks further recognition of the CAP1400 by the international authority, laying a solid foundation for the CAP1400 to participate in international competition at a higher level."

International use of the CAP1400 is still dependent on meeting country-specific standards and requirements, but passing the IAEA safety review will make this process easier.

In September 2014, the Chinese nuclear regulator approved the preliminary safety analysis report of the CAP1400 reactor design following a 17-month review. The National Nuclear Safety Administration's safety review involved more than 260 experts, 30 meetings to discuss it and responding to more than 5000 questions, according to SNPTC. As a result of the review, more than 1000 work orders were drawn up.

Site preparation is already underway for two demonstration CAP1400 units at Huaneng Group's Shidaowan site in Shandong province. The pouring of first concrete is expected to take place soon.

Researched and written
by World Nuclear News
 
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Building compact particle accelerators: Bunching electrons can get more done
Researchers propose a new method to improve beam quality in plasma wakefield accelerators by getting the electrons to travel at the same speed

Date: May 10, 2016
Source: American Institute of Physics

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Researchers propose a new method to improve plasma wakefield accelerators by compressing the electron beam. Simulations show two-dimensional electron density distribution for the injector stage (A), compressor stage (B) and accelerator stage (C), where the target e-beam is circled by a dashed circle (in red).
Credit: Jiansheng Liu/Chinese Academy of Sciences

In the world of particle accelerators, laser wakefield devices are the small, but mighty upstarts. The machines can accelerate electrons to near the speed of light using a fraction of the distance required by conventional particle accelerators. However, the electrons are not all uniformly accelerated and beams with a mix of faster (higher energy) and slower (lower energy) particles are less practical.

Now a team of researchers from China, South Korea and the U.S. has proposed a new way to minimize the energy spread of electrons in laser wakefield accelerators. They publish their method in the journal Physics of Plasmas, from AIP Publishing.



Full Story -> Building compact particle accelerators: Bunching electrons can get more done: Researchers propose a new method to improve beam quality in plasma wakefield accelerators by getting the electrons to travel at the same speed -- ScienceDaily

Journal Reference:
Zhijun Zhang, Wentao Li, Jiansheng Liu, Wentao Wang, Changhai Yu, Ye Tian, Kazuhisa Nakajima, Aihua Deng, Rong Qi, Cheng Wang, Zhiyong Qin, Ming Fang, Jiaqi Liu, Changquan Xia, Ruxin Li and Zhizhan Xu. Energy spread minimization in a cascaded laser wakefield accelerator via velocity bunching. Physics of Plasmas, 2016 DOI: 10.1063/1.4947536
 
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Manned submersible’s mother ship arrives in Sanya
Updated: May 11,2016 11:44 AM english.gov.cn

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The Tansuo-1 research vessel lies at anchor in Sanya port, May 10, 2016.[Photo/Xinhua]

Modifications for China’s first manned submersible capable of reaching 4,500 meters and its mother ship, Tansuo-1, were completed on May 5 before arriving at Sanya in South China’s Hainan province on May 8. With a length of 94.45 meters and width of 17.9 meters, the Tansuo-1 has a full-load displacement of 6,250 tons. As the mother ship of the manned submersible, Tansuo-1 will be on sea trials and deep-sea operation of a manned and unmanned submersible capable of diving to depths of 10,000 meters.

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The Tansuo-1 research vessel comes equipped with an A-shaped frame with a lifting capacity of 150 tons.[Photo/Xinhua]

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The Tansuo-1’s 13,000-meter cable will be used for expedition missions, including geological sampling.[Photo/Xinhua]

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The manned submersible will be stored in this area. [Photo/Xinhua]

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A staffer operates the Tansuo-1 research vessel on May 10, 2016.[Photo/Xinhua]



Published on May 10, 2016
The mother ship for China's new deep-sea submersible arrived in the southern Chinese port of Sanya on Tuesday.

The 94-meter "Tansuo-1" was delivered to its owner, the Chinese Academy of Sciences, or CAS, a day earlier.

It has a full-load displacement of 6,250 tonnes, and has an range of 10,000 nautical miles.

It's equipped with 10 permanent research labs and two removable labs.

The ship will serve as the mother ship for a new submersible currently under development and for future CAS expeditions of sea floor trenches.

It will conduct off-shore tests in waters near Sanya.

If all goes well, the ship will sail on to the Mariana Trench in June for a research mission.

The new manned submersible that can reach a depth of 4,500 meters is likely to go through off-shore testing in the first half of next year.

China's current manned submersible "Jiaolong" reached a depth of 7,062 meters in the Mariana Trench in June 2012.
 
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Chinese team cracks Zika secret

By Zhao Xinying/Cheng Yingqi (China Daily)
Updated: 2016-05-12 07:08

Research shows a link between the virus, poor brain development

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Researcher Xu Zhiheng explains findings of a Chinese team studying the Zika virus in Beijing on Wednesday. [Photo by Zou Hong/China Daily]


A research finding by Chinese scientists that was published in a leading academic journal identified a direct link between the Zika virus and microcephaly-a disorder in which the head is small due to a defect in brain development.

The research was a collaboration between Xu Zhiheng, principal investigator at the Institute of Genetics and Developmental Biology under the Chinese Academy of Sciences, and Qin Chengfeng, a professor of virology at the Institute of Microbiology and Epidemiology under the Academy of Military Medical Sciences.

The authors published their findings on Wednesday in Cell Stem Cell, a monthly journal that focuses on important findings in stem cell research. They showed that mouse fetuses injected with the Asian Zika virus that were carried to term displayed characteristic features of microcephaly.

As expected, the virus infected neural progenitor cells, and the brains revealed genetic signs of viral entry, altered immune response and cell death. The authors said that's direct evidence that Zika infection causes microcephaly in a mammal.

The Zika virus has broken out in South America and been spreading around the world since 2015-including China.

"Zika is not a newly discovered virus, but its breakout in 2015 attracted more attention than ever before because more than 6,000 babies born in Brazil from March 2015 to March 2016 were diagnosed with microcephaly. Most of these babies' mothers were infected by the Zika virus," Qin said.

Until now, no direct connection between Zika and microcephaly has been found. According to Xu, mutations of about 30 human genes could cause microcephaly.

Recently, a research team of scientists from the United States and Brazil published a finding in Nature, showing that the Brazilian Zika virus strain could lead to birth defects.

But the Chinese research went further, Xu said. The most surprising finding was that it was mostly neural progenitor cells that became infected at the beginning, and mostly neurons infected at a later stage, five days after injection, when the presence of Zika virus increases several hundredfold.

"However, almost all cell deaths were found in neurons other than neural progenitor cells," Xu said. "This indicates that neurons, not neural progenitor cells, are prone to induced cell death by the Zika virus."

Xu said he hoped the finding would lay a good foundation for further research and control of the virus. The animal model, together with the global data sets of infected brains, "will provide valuable resources for further investigation", he said.

Symptoms generally mild

Zika is a virus that is primarily spread by mosquito bites. In general, the symptoms known to be caused by the virus tend to be mild. They include fever, rash, pain in the joints and pink eye. Symptoms usually occur two to seven days after infection. Many people who are infected do not show any symptoms, and those who do can be treated easily. Zika was first found in Africa and spread to Asia and Latin America. The virus is spreading rapidly in Latin America, while Thailand and the Philippines are the most Zika-infected countries in Asia.

http://www.chinadaily.com.cn/china/2016-05/12/content_25224690.htm
 
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First deep-sea research institute opens in Sanya
By Shan Jie Source:Global Times Published: 2016-5-12 0:48:01

China's first deep-sea research institute has opened in Sanya, South China's Hainan Province, which experts said will be meaningful for resource exploitation and improvements to naval technology.

The Institute of Deep-Sea Science and Engineering (IDSSE) at the Chinese Academy of Sciences (CAS) passed an acceptance inspection and began official operations on Tuesday, the China News Agency reported Wednesday.

The IDSSE is the first scientific research base for study of the deep seas and is also China's first public platform for deep-sea research and technological experiments, media reported.

"The deep sea has a wealth of resources including mineral, biotic and petroleum resources, and there are more than 40 billion tons of petroleum in the South China Sea alone. Therefore, it is necessary to enhance scientific research capabilities there," Gao Shu, director of the School of Geographic and Oceanographic Sciences at Nanjing University, told the Global Times on Wednesday.

Since 2012, Chinese scientists with the CAS have participated in several research projects related to the South China Sea, including scientific voyages in the area, according to the CAS website.

"China is a big country both in land and in sea, but in the past China has put emphasis on development on land, so [the opening of] the institute shows that the exploitation of ocean resources in China has arrived at a new stage," said Wang Xiaopeng, an expert on maritime and border studies at the CAS.

Wang added that scientific research on the use of marine resources has been a hot topic in many countries since the release of the UN Convention on the Law of the Sea in 1982, which reallocated many global ocean resources.

Deep-sea research will also have military uses, as studies on complex marine environments can help improve naval technology, which is usually the most advanced military technology in a country, Gao added.

The mother ship for a new deep-sea submersible was delivered to the CAS in Guangzhou, South China's Guangdong Province on May 5. The 94-meter Tansuo 1 has a fully loaded displacement of 6,250 tons, and the submersible can reach a depth of 4,500 meters. It is expected to dive to the Mariana Trench for a research mission in the near future, according to the Xinhua News Agency.
 
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Public Release: 11-May-2016
Fetal mice with Zika infection get microcephaly
Cell Press

Mouse fetuses injected with the Asian Zika virus strain and carried to term within their pregnant mothers display the characteristic features of microcephaly, researchers in China report May 11 in Cell Stem Cell. As expected, the virus infected the neural progenitor cells, and infected brains reveal expression of genes related to viral entry, altered immune response, and cell death. The authors say this is direct evidence that Zika infection causes microcephaly in a mammalian animal model.

The research was a collaborative effort between Zhiheng Xu at the Institute of Genetics and Developmental Biology of the Chinese Academy of Sciences and Cheng-Feng Qin at the Beijing Institute of Microbiology and Epidemiology.


Full Story -> Fetal mice with Zika infection get microcephaly | EurekAlert! Science News
 
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China builds largest metal 3D printing equipment
New China TV

Published on May 9, 2016
China has built a new metal 3D printing equipment, which is recognized as the largest such facility in the world.
 
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China's first mid-long range emergency supplies transporting drone successfully completed its maiden flight on Tuesday.

The fixed-wing drone can carry 15 kilograms of goods on flight of up to 80 kilometers and make accurate delivery within a range of 15 meters, marking a breakthrough in terms of mileage, load, and short distance of take-off and landing compared with other drones available at the moment.

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Consumer-friendly smart camera will follow you around and record your life
(People's Daily Online) 14:43, May 13, 2016

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Nowadays, people all over the world employ selfie sticks to document exciting moments in their lives. But now there’s a new tool in town!

Hover Camera, developed by Beijing startup Zero Zero Robotics, has attracted media coverage after launching its product display in the U.S. The camera resembles a computer hard drive and is portable because its wings can fold up.

Hover Camera can independently follow a moving subject and knows how to avoid other objects. As the name suggests, the new product is being used mainly for aerial photography and videography, but one thing that differentiates it from other drones is the fact that its propellers are fully enclosed by a strong carbon fiber frame, making it much safer than other models on the market.

The functions of Hover Camera are very impressive. There's the 360 Pano mode, along with face tracking and body tracking. The device utilizes a Snapdragon 801 processor and only weighs 240 grams. The main camera in the front takes 13-megapixel stills as well as 4K video, and it comes with a dual-tone flash. Since it's only on a single-axis gimbal, it offers electronic image stabilization. There's also a downward-facing 3-megapixel camera and sonar underneath to help stabilize the drone itself.

It will cost around $600 when it goes on sale this summer.

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Wang Mengqiu, founder and CEO of Zero Zero Robotics, was born in Hangzhou in 1982. He has a PhD from Stanford University and has worked for Facebook, Alibaba and Twitter.
 
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中國造出世界最大起重船:重達14萬噸(圖)
China create the world's largest crane vessel: weighing 140,000 tons.


更新時間: 2016/5/14 9:46:00
Updated: 2016/5/14 9:46:00​

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5月13日,振華重工自主建造的世界最大12000噸起重船在上海長興島基地交付,並在現場命名為“振華30號”。
May 13, Zhenhua Heavy Industry independently-constructed the world's largest crane ship delivered in Shanghai Changxing base, and named "Zhenhua 30" on the spot.
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“振華30號”
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2000噸起重船的問世,對當今世界打撈行業可謂恰逢其時。​

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Actual photo of Zhen Hua 30 lifting 13,200MT, a world record set in a lifting test that occurred in Houston, Texas on 26 January 2016.

The company that receive the new vessel is reported to be contracted for salvaging vessel MV Sewol, a Korean vehicle-passenger ferry that sunk off the coast of Korea on April 16, 2014 that killed 295 passengers, mostly student.

https://en.wikipedia.org/wiki/Sinking_of_MV_Sewol
 
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SMIC Again Boosts 2016 Capex to $2.5 Billion

Patterson

5/13/2016 10:21 AM EDT

TAIPEI—Semiconductor Manufacturing International Corp. (SMIC), China’s largest foundry, today said it will increase its capital expenditure budget for 2016 to $2.5 billion on expectations that strong demand will continue for another four years.

Three months ago, SMIC boosted 2016 capex to $2.1 billion from $1.57 billion last year to capture more business in China, the world’s fastest growing chip market.

Reiterating its expectations for a 20% increase in its 2016 sales from $2.24 billion in 2015, SMIC forecast that it is targeting annual sales growth of 20% for the next three to four years.

“We are embarking on a new phase of growth,” SMIC CEO Tzu-Yin Chiu said on a conference call to announce the company’s first-quarter 2016 results. “We remain constrained by the pace of capacity growth.”

(Source: SMIC)

The company is uncharacteristically bullish as growth in the global semiconductor industry this year slumps amid an inventory glut. SMIC said its first-quarter sales revenue reached a record high of $634.3 million as its fabs continued to run at full utilization.

While its largest competitors such as Taiwan Semiconductor Manufacturing Co. (TSMC) and Samsung are selling leading-edge 16nm and 14nm products to companies such as Apple and Qualcomm, SMIC has been making gross margins of about 25% by selling chips made with 28nm and 40nm as its most advanced process technology.

In April, TSMC reiterated its forecast for its 2016 capex to be within a range of $9 billion to $10 billion as it aims for a bigger share of finer geometry chips.

SMIC plans to use its increased capex budget primarily to ramp up 40nm in a new Beijing fab this year while preparing to boost 28nm output starting next year.

“Twenty-eight nanometer demand is strong,” Chiu said. “Forty nanometer demand is much stronger.”

By the fourth quarter of this year, SMIC expects sales of products made with 40nm and finer process technology to more than double from a year ago. The company’s plan to increase 40nm production this year will result in 28nm revenue falling within a range of 5% to 8% of total sales, contrary to its earlier forecast this year for 28nm to account for a “double-digit” portion of company revenue by the end of the fourth quarter.

Currently, SMIC said it has one major customer producing “high volumes” of 28nm chips and an additional four customers that are preparing to launch 28nm products.

New business

SMIC is ramping up 40nm to help an unidentified customer launch a new product during the second half of this year. Some of the key drivers for 40nm demand are WiFi, digital TV, set-top boxes and radio-frequency applications, the company said.

During the first quarter, China was SMIC’s largest source of sales revenue by geography. China’s portion of SMIC sales rose to 47.2% in the first quarter from 45% in the fourth quarter last year.

—Alan Patterson covers the semiconductor industry for EE Times. He is based in Taiwan.
 
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05.13.2016 13:51
China Plans World's Largest Particle Collider to Unlock Universe's Mysteries
Supporters say the billion dollar facility will help improve understanding of the origins of matter, but critics warn it could be more expensive than planned

By staff reporters Zhang Yan and Yu Dawei


(Beijing) – China plans to invest US$ 6 billion to build the world's largest particle collider to get a foot in the door of experimental physics dominated by European and American research labs, but some scientists warn it could be a wasteful undertaking.

The blueprint for what scientists call a super collider, an underground facility to smash subatomic particles at high speeds, was drafted in 2014 by scientists at the Institute of High Energy Physics (IHEP) in Beijing. The project, dubbed the "Higgs Factory," aims to build a facility capable of generating millions of Higgs boson particles, which physicists say form the building blocks of the universe.

Scientists at IHEP have completed the design for an underground ring with a circumference of 50 to 100 kilometers that can smash together electrons and positrons, state media reported in October. If built, it would be at least twice the size of the world's largest particle accelerator, a 27-kilometer circular tunnel beneath the Swiss-French border.

The plan is yet to be approved by the central government, but scientists are optimistic that research needed to build the facility can start in late 2016. Construction of the first phase of this Circular Electron Positron Collider is expected to begin in 2021 near Qinhuangdao, a port city in the northern province of Hebei, scientists said.

The second phase of the project, which will upgrade the facility to a proton–proton collider, is set to start by 2040.

The lab could help the country "leap to a leadership position in an important frontier in basic science," wrote David Gross, an American theoretical physicist and Nobel laureate, in a commentary in the Wall Street Journal in September.

The project "would transport physics into a previously inaccessible high-energy realm," wrote Harvard professor Shing-Tung Yau and science journalist Steve Nadis in their book, From the Great Wall to the Great Collider, published in April.

But the plan has hit a snag, with several scientists warning the project might be an unrealistic and wasteful endeavor. The government has also been cautious. It has not given any feedback on IHEP's proposal so far, sources close to the project said.

Doubtful Leap

The discovery of the Higgs boson particle in 2012 was seen by experts as one of the biggest scientific breakthroughs in human history, changing the way we understand our universe, how it originated and its future.

It was found by a team of scientists experimenting at the Large Hadron Collider (LHC) by crashing high-energy proton beams at velocities near the speed of light. This research facility, operated by the European Organization for Nuclear Research, is known as CERN.

However, the accelerator may not be able to generate large quantities of Higgs boson particles to support further studies, said IHEP director Wang Yifang in a 2015 interview with state-run newspaper China Daily.

The "LHC is hitting its limits in terms of energy levels (needed to smash particles)," said Wang. "It seems that it is not possible to escalate the energy (level) dramatically at the existing facility."

CERN said in October that it was working on improving its facilities by 2025.

China's particle accelerator promises to go a step further in unlocking the mysteries of the universe. It will operate at about seven times the energy level of the LHC, said Wang, and will be able to generate large quantities of Higgs boson particles to try to recreate the conditions that followed the Big Bang, one theory that explains the origins of the universe and matter.

Critics, however, doubt this plan. Some theories have proved that the study of particles using high energy collision experiments has almost reached its limits, said Cao Zexian, a physicist at the Chinese Academy of Sciences. "Nobody can be sure that high energy colliders can make new discoveries," and that is why no other country has proposed the construction of a new collider, he said.

Wang says there are several areas in the field of high energy physics that still need to be explored. Countries like the United States are hesitating to build new facilities due to cost concerns amid an economic slowdown, he said.

In the 1980s, the United States started to build what was called a Superconducting Super Collider with a ring circumference of 87.1 kilometers, but the project in the state of Texas was called off in 1993 due to rising costs.

Although many physicists say they want a bigger facility for further research, sluggish economic growth in the United States and debt woes in Europe are preventing governments from investing large sums to develop the field of high energy physics, Michael Riordan, professor of physics at University of California, Santa Cruz, said.

IHFP operates a 220 meter electron-positron collider in Beijing, the largest one in the country, built in 1990.

Chinese authorities may be waiting for more research to show that high energy physics is a promising field of research to invest in. In December 2015, scientists at CERN said the LHC might have discovered a new particle, which cannot be defined using existing laws of physics.

Researchers are expected to publish detailed findings about the new particle by the end of the year, said Xu Cenke, professor of physics at the University of California, Santa Barbara, and their findings will serve as an important reference when Chinese authorities decide whether to go ahead with plans to build a domestic lab.

Weighing the Benefits

The research needed to build the collider will improve technology used in several industries, Wang aid. Some areas that can benefit are cryocooler technology, a cooling technique that can be used to preserve human cells and organs, designing precision machines used on assembly lines and discovering new material to build semiconductors for computer chips, he said.

"Every dollar we spend on the collider will help improve the country's technology capacity," said Wang. Most of the components needed to build the facility will be made domestically by local scientists, he said.

But Cao from the Chinese Academy of Sciences said key components and technologies needed for the project will have to be imported because the country's high-tech research and precision machine development capabilities still lag behind those of developed countries. Most of the money will be spent on buying imported equipment and parts, he said.

Some experts worry that the costs could rise way above the estimated budget. When the LHC was completed, the final bill stood at US$ 9 billion, more than three times the initial budget of US$ 2.6 billion. It also costs more than US$ 1 billion every year to operate and maintain the facility.

Opponents of the projects argued that instead of building a larger collider, the money should be spent on developing other fields of research with a more immediate social impact.

"It is hard to say whether it is right or wrong to invest so much to build a collider," said Cao. "But before that, we can try to improve our computer chips and the tips of ballpoint pens."

(Rewritten by Han Wei)

http://english.caixin.com/2016-05-13/100943237.html
 
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World's largest floating single crane ship delivered in Shanghai
New China TV

Published on May 16, 2016
The world's largest floating single crane ship has been delivered in east China's Shanghai.
 
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Mirror-image enzyme copies looking-glass DNA
Synthetic polymerase is a small step along the way to mirrored life forms.
  • Mark Peplow
  • 16 May 2016
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Zixuan Li, Xin Tao, Ting F. Zhu
The DNA in our cells twists like a right-handed screw (pictured, bottom); a mirror-image polymerase enzyme is needed to copy left-handed DNA (pictured, top).

Researchers at Tsinghua University in Beijing have created a mirror-image version of a protein that performs two of the most fundamental processes of life: copying DNA and transcribing it into RNA.

The work is a “small step” along the way to making mirror-image life forms, says molecular biologist Jack Szostak of Harvard Medical School in Boston, Massachusetts. “It’s a terrific milestone,” adds his Harvard colleague George Church, who hopes one day to create an entire mirror-image cell.


Many organic molecules are ‘chiral’: that is, they can exist in mirror-image forms that cannot be superimposed, like a right-handed and left-handed glove. But life almost always employs one version: cells use left-handed amino acids, and have DNA that twists like a right-handed screw, for instance.

In principle, looking-glass versions of these molecules should work together in the same way as normal ones — but they might be resistant to attack by conventional viruses or enzymes that have not evolved in a looking-glass world.

That makes mirror-image biochemistry a potentially lucrative business. One company that hopes so is Noxxon Pharma in Berlin. It uses laborious chemical synthesis to make mirror-image forms of short strands of DNA or RNA called aptamers, which bind to therapeutic targets such as proteins in the body to block their activity. The firm has several mirror-aptamer candidates in human trials for diseases including cancer; the idea is that their efficacy might be improved because they aren’t degraded by the body’s enzymes. A process to replicate mirror-image DNA could offer a much easier route to making the aptamers, says Sven Klussmann, Noxxon Pharma’s chief scientific officer.

Through the looking-glass
Researchers have been making chunks of mirror-DNA for decades, so the Tsinghua team could order much of what they needed for their looking-glass DNA replication attempt from a chemical supplier: a mirror-DNA strand to be copied, mirror-DNA building blocks and a shorter mirror ‘primer’ strand that could pick up these building blocks in the right order.

The difficult task was to make the mirror-image enzyme that coordinates the copying process, called DNA polymerase. That would need to be synthesized from right-handed amino acids, but commonly used polymerase enzymes have more than 600 amino acids — meaning that they are too big for current synthetic methods.

So the Tsinghua team turned to the smallest known polymerase: African swine fever virus polymerase X, which contains just 174 amino acids. Unfortunately, it is also spectacularly slow — probably because of its small size, says synthetic biologist Ting Zhu, a former graduate student of Szostak’s who helped to lead the work. The team made a mirror version of the enzyme and found that, like its natural equivalent, it could extend a mirror-primer consisting of 12 nucleotides (DNA building blocks) to an 18-nucleotide mirror-DNA strand in about four hours; and to a 56-nucleotide strand in 36 hours.

When the normal and mirror-image versions of these systems were mixed together in the same test tube, both replication processes worked independently without interference. The mirror-image polymerase could also transcribe mirror-DNA into mirror-RNA, again at a glacial pace. The work is published in Nature Chemistry.

Klussmann says that Noxxon Pharma is interested in pursuing a similar approach with a more efficient enzyme. Indeed, Zhu and his colleagues next hope to build a mirror-image of a more efficient polymerase known as Dpo4, which is built of 352 amino acids.

Life, backwards
In their research paper, the Tsinghua researchers also present their work as an effort to investigate why life’s chirality is the way it is. This remains mysterious: it may simply be down to chance, or it could have been triggered by a fundamental asymmetry in nature.

But Steven Benner, at the Foundation for Applied Molecular Evolution in Alachua, Florida, says it’s unlikely that creating a mirror form of biochemical life could shed any light on this question. Almost every physical process behaves identically when viewed in a mirror. The only known exceptions — called ‘parity violations’ — lie in the realm of subatomic physics. Such tiny differences would never show up in these biochemical experiments, says Benner. (He is also interested in making DNA that can avoid unwanted degradation by natural enzymes or viruses, but rather than using mirror-DNA, he has created artificial DNA with non-natural building blocks.)

Church’s ultimate goal, to make a mirror-image cell, faces enormous challenges. In nature, RNA is translated into proteins by the ribosome, a complex molecular machine. “Reconstructing a mirror-image of the ribosome would be a daunting task,” says Zhu. Instead, Church is trying to mutate a normal ribosome so that it can handle mirror-RNA.

Church says that it is anyone’s guess as to which approach might pay off. But he notes that a growing number of researchers are working on looking-glass versions of biochemical processes. “For a while it was a non-field,” says Church. “But now it seems very vibrant.”

Nature
doi:10.1038/nature.2016.19918

References

Wang, Z., Xu, W., Liu, L. & Zhu, T. F. Nature Chem. http://dx.doi.org/10.1038/nchem.2517 (2016).​


Mirror-image enzyme copies looking-glass DNA : Nature News & Comment
 
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