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Scientists Visualize Structure of Key DNA Repair Component with ‘Near-Atomic Resolution’
[2017-11-30]
Cells continuously replicate to repair and replace damaged tissue, and each division requires a reprinting of the cell’s genetic blueprints. As the DNA duplicates, errors inevitably occur, resulting in damage that, if left unrepaired, can lead to cellular death. At the first hint of DNA damage, a protein known as an ATR kinase activates the cell’s built-in repair system. Scientists have now imaged this protein at unprecedented resolution, and are beginning to understand its response to DNA damage.
Artist's concept of Launching the cellular DNA-damage response by the ATR-ATRIP complex. /By WANG Guoyan and CHEN Lei, USTC
The researchers published the structural information today in Science.
“The ATR protein is the apical kinase to cope with the DNA damages and replication stress,” said CAI Gang, a professor of life sciences at the University of Science & Technology of China in Hefei, China, and the lead author on the paper. “It has long been a central question to determine the activation mechanism of ATR kinase—how it responds to DNA damage and how it is activated.”
CAI and his team used electron microscopy to image the Mec1-Ddc2 complex at 3.9 ångströms, which is about eight times the size of a single atom of helium. The complex is found in yeast and is the equivalent of the human ATR protein and its cell-signaling protein partner, ATRIP.
The ATR kinase is one of six proteins responsible for maintaining the health of the cell. When this family of proteins identify a problem, such as DNA damage, they instigate the downstream signals needed to repair the damage.
“Cryo-electron microscopy of the Mec1-Ddc2 with state-of-the-art instrumentation has resulted in an electron density map at near-atomic resolution,” said CAI, noting that the improved map has confirmed and expanded upon previous findings.
Three-dimensional structure of the yeast Mec1-Ddc2 complex, a homolog of human ATR-ATRIP. /By WANG Guoyan and MA Yanbing, USTC
ATR has long been a potential therapeutic target, according to CAI. The high-resolution structural information revealed regulatory sites of the ATR kinase, which are poised to activate at the first hint of DNA damage. Elucidating this mechanism could aid in the development of new therapeutics.
“The structure of the yeast member closely resembles those of the human counterpart,” said CAI, drawing attention to the substantial similarity in the detailed architecture. “We believe the information acquired from the yeast Mec1-Ddc2 shed light on the architecture and mechanism of the human ATR-ATRIP complex.”
Scientists Visualize Structure of Key DNA Repair Component with ‘Near-Atomic Resolution’ | USTC Highlights
Xuejuan Wang, Tingting Ran, Xuan Zhang, Jiyu Xin, Zhihui Zhang, Tengwei Wu, Weiwu Wang, Gang Cai. 3.9 Å structure of the yeast Mec1-Ddc2 complex, a homolog of human ATR-ATRIP. Science (2017). DOI: 10.1126/science.aan8414
Ancient flying reptiles cared for their young, fossil trove suggests
By Gretchen Vogel
Nov. 30, 2017 , 2:00 PM
A spectacular fossil find is providing tantalizing new clues about the habits of pterosaurs, ancient flying reptiles that lived at the same times as dinosaurs. The cache of more than 200 fossil eggs found with bones of juvenile and adult animals in northwestern China is “one of the most extraordinary fossil [finds] I’ve ever seen,” says David Unwin, a paleontologist at the University of Leicester in the United Kingdom, who was not involved in the work. And it suggests to some researchers that pterosaur parents may have cared for their newly hatched young.
The fossils formed about 120 million years ago when disaster struck a group of pterosaurs. The researchers speculate that when a sudden rain flooded a river, hundreds of pterosaur eggs buried in shallow sand or under a layer of leaves or grass were drowned and washed downstream, along with a number of older individuals. Quickly buried by sediment, the eggs and bones did not decay but instead were preserved as fossils. “You’ve captured the life history of pterosaurs,” Unwin says.
Only a few fossilized pterosaur eggs had turned up before, at sites in Argentina and in China. But in a paper published today in Science, Wang Xiaolin and Jiang Shunxing at the Chinese Academy of Sciences’s Institute of Vertebrate Paleontology and Paleoanthropology in Beijing and their colleagues report that a 3-meter square chunk of rock they excavated in the Turpan-Hami Basin in northwest China contains more than 200 eggs of the pterosaur, called Hamipterus tianshanensis. In 16 of them, researchers have been able to identify fossilized bones of developing embryos.
Whatever transported the eggs to their resting place likely damaged them, so the bones are jumbled and incomplete. But enough is preserved to allow comparisons between the bones in the embryos and those of older pterosaurs also preserved, says Alexander Kellner of the National Museum at the Federal University of Rio de Janeiro in Brazil, who helped analyze the fossils. “It’s amazing,” he says. “We never thought we would find so many eggs.”
This Chinese fossil contains hundreds of pterosaur eggs and bones.
Wang et al., Science, 2017
The researchers used computerized tomography scans to measure some of the embryonic bones and took thin slices of some to tell how mature they were. In one particularly well preserved egg, the hind limbs were more developed than the forelimbs. That suggests, Kellner says, that pterosaurs could walk when they hatched, but not fly. The embryos also appeared to be toothless, unlike some dinosaur embryos. Together, the authors say, the evidence suggests that hatchlings might have not been able to hunt for themselves, relying on their parents to feed them. “They needed some sort of parental care,” Kellner says.
Unwin says he’s not yet convinced. The smallest hatchlings in the sample are 40% bigger than the embryos, he notes, so the forelimbs might have matured by the time they hatched. Charles Deeming, an expert on reptile reproduction at the University of Lincoln in the United Kingdom, also cautions about drawing firm conclusions from close analysis of just a few eggs. Although fossil egg finds are spectacular, he says, “one of the dangers … is that they are often overinterpreted.”
Jiang says that’s a fair critique, but he and others say further analysis of the wealth of eggs at the site will eventually provide firmer evidence one way or another. “The numbers [of eggs and bones] mean that we can move on from positing ideas to testing ideas,” Unwin says. Pterosaur remains scattered through multiple layers of the rocks suggest that the site was a pterosaur nesting site for many years. “It must have been a great place to bury eggs,” Unwin says—until, periodically, catastrophe struck. The specimen described today is only the start, Jiang says. “There are many more eggs.”
Ancient flying reptiles cared for their young, fossil trove suggests | Science | AAAS
###Scientists just discovered the mother lode of pterosaur eggs, and they are over the moon
By Jason Bittel November 30 at 2:00 PM
“Extraordinary.” “Stellar.” “Truly awesome.” “A world-class find.”
That's how paleontologists are reacting to the discovery of several hundred ridiculously well-preserved pterosaur eggs in China, some of them still containing the remains of embryos.
“My first thought was extreme jealousy,” said David Unwin, a pterosaur expert and paleobiologist at the University of Leicester. “Really.”
To understand why Unwin and others are freaking out about the discovery, published Thursday in the journal Science, you have to first appreciate how rare pterosaur eggs are.
--> Scientists just discovered the mother lode of pterosaur eggs, and they are over the moon - The Washington Post
Xiaolin Wang, Alexander W. A. Kellner, Shunxing Jiang, Xin Cheng, Qiang Wang, Yingxia Ma, Yahefujiang Paidoula, Taissa Rodrigues, He Chen, Juliana M. Sayão, Ning Li, Jialiang Zhang, Renan A. M. Bantim, Xi Meng, Xinjun Zhang, Rui Qiu, Zhonghe Zhou. Egg accumulation with 3D embryos provides insight into the life history of a pterosaur. Science (2017). DOI: 10.1126/science.aan2329
No idea.One thing I don't understand is why the Chinese scientist collaborated with Brazilians in this paper.
Any insights?
No idea.
200 eggs are a crazy find.Ancient flying reptiles cared for their young, fossil trove suggests
By Gretchen Vogel
Nov. 30, 2017 , 2:00 PM
A spectacular fossil find is providing tantalizing new clues about the habits of pterosaurs, ancient flying reptiles that lived at the same times as dinosaurs. The cache of more than 200 fossil eggs found with bones of juvenile and adult animals in northwestern China is “one of the most extraordinary fossil [finds] I’ve ever seen,” says David Unwin, a paleontologist at the University of Leicester in the United Kingdom, who was not involved in the work. And it suggests to some researchers that pterosaur parents may have cared for their newly hatched young.
The fossils formed about 120 million years ago when disaster struck a group of pterosaurs. The researchers speculate that when a sudden rain flooded a river, hundreds of pterosaur eggs buried in shallow sand or under a layer of leaves or grass were drowned and washed downstream, along with a number of older individuals. Quickly buried by sediment, the eggs and bones did not decay but instead were preserved as fossils. “You’ve captured the life history of pterosaurs,” Unwin says.
Only a few fossilized pterosaur eggs had turned up before, at sites in Argentina and in China. But in a paper published today in Science, Wang Xiaolin and Jiang Shunxing at the Chinese Academy of Sciences’s Institute of Vertebrate Paleontology and Paleoanthropology in Beijing and their colleagues report that a 3-meter square chunk of rock they excavated in the Turpan-Hami Basin in northwest China contains more than 200 eggs of the pterosaur, called Hamipterus tianshanensis. In 16 of them, researchers have been able to identify fossilized bones of developing embryos.
Whatever transported the eggs to their resting place likely damaged them, so the bones are jumbled and incomplete. But enough is preserved to allow comparisons between the bones in the embryos and those of older pterosaurs also preserved, says Alexander Kellner of the National Museum at the Federal University of Rio de Janeiro in Brazil, who helped analyze the fossils. “It’s amazing,” he says. “We never thought we would find so many eggs.”
This Chinese fossil contains hundreds of pterosaur eggs and bones.
Wang et al., Science, 2017
The researchers used computerized tomography scans to measure some of the embryonic bones and took thin slices of some to tell how mature they were. In one particularly well preserved egg, the hind limbs were more developed than the forelimbs. That suggests, Kellner says, that pterosaurs could walk when they hatched, but not fly. The embryos also appeared to be toothless, unlike some dinosaur embryos. Together, the authors say, the evidence suggests that hatchlings might have not been able to hunt for themselves, relying on their parents to feed them. “They needed some sort of parental care,” Kellner says.
Unwin says he’s not yet convinced. The smallest hatchlings in the sample are 40% bigger than the embryos, he notes, so the forelimbs might have matured by the time they hatched. Charles Deeming, an expert on reptile reproduction at the University of Lincoln in the United Kingdom, also cautions about drawing firm conclusions from close analysis of just a few eggs. Although fossil egg finds are spectacular, he says, “one of the dangers … is that they are often overinterpreted.”
Jiang says that’s a fair critique, but he and others say further analysis of the wealth of eggs at the site will eventually provide firmer evidence one way or another. “The numbers [of eggs and bones] mean that we can move on from positing ideas to testing ideas,” Unwin says. Pterosaur remains scattered through multiple layers of the rocks suggest that the site was a pterosaur nesting site for many years. “It must have been a great place to bury eggs,” Unwin says—until, periodically, catastrophe struck. The specimen described today is only the start, Jiang says. “There are many more eggs.”
Ancient flying reptiles cared for their young, fossil trove suggests | Science | AAAS
###Scientists just discovered the mother lode of pterosaur eggs, and they are over the moon
By Jason Bittel November 30 at 2:00 PM
“Extraordinary.” “Stellar.” “Truly awesome.” “A world-class find.”
That's how paleontologists are reacting to the discovery of several hundred ridiculously well-preserved pterosaur eggs in China, some of them still containing the remains of embryos.
“My first thought was extreme jealousy,” said David Unwin, a pterosaur expert and paleobiologist at the University of Leicester. “Really.”
To understand why Unwin and others are freaking out about the discovery, published Thursday in the journal Science, you have to first appreciate how rare pterosaur eggs are.
--> Scientists just discovered the mother lode of pterosaur eggs, and they are over the moon - The Washington Post
Xiaolin Wang, Alexander W. A. Kellner, Shunxing Jiang, Xin Cheng, Qiang Wang, Yingxia Ma, Yahefujiang Paidoula, Taissa Rodrigues, He Chen, Juliana M. Sayão, Ning Li, Jialiang Zhang, Renan A. M. Bantim, Xi Meng, Xinjun Zhang, Rui Qiu, Zhonghe Zhou. Egg accumulation with 3D embryos provides insight into the life history of a pterosaur. Science (2017). DOI: 10.1126/science.aan2329
Brazil is an important supplier of iron ore to China.One thing I don't understand is why the Chinese scientist collaborated with Brazilians in this paper.
Any insights?